Mechanisms underlying lactic acid tolerance and its influence on lactic acid production in Saccharomyces cerevisiae
نویسندگان
چکیده
منابع مشابه
Changes in SAM2 expression affect lactic acid tolerance and lactic acid production in Saccharomyces cerevisiae
BACKGROUND The great interest in the production of highly pure lactic acid enantiomers comes from the application of polylactic acid (PLA) for the production of biodegradable plastics. Yeasts can be considered as alternative cell factories to lactic acid bacteria for lactic acid production, despite not being natural producers, since they can better tolerate acidic environments. We have previous...
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A plant- and crop-based renewable plastic, poly-lactic acid (PLA), is receiving attention as a new material for a sustainable society in place of petroleum-based plastics. We constructed a metabolically engineered Saccharomyces cerevisiae that has both pyruvate decarboxylase genes (PDC1 and PDC5) disrupted in the genetic background to express two copies of the bovine L-lactate dehydrogenase (LD...
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Yeast strains expressing heterologous L-lactate dehydrogenases can produce lactic acid. Although these microorganisms are tolerant of acidic environments, it is known that at low pH, lactic acid exerts a high level of stress on the cells. In the present study we analyzed intracellular pH (pHi) and viability by staining with cSNARF-4F and ethidium bromide, respectively, of two lactic-acid-produc...
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Lactic acid is widely used in the food, cosmetic, pharmaceutical, and chemical industries and has received increased attention for use as a monomer for the production of biodegradable poly(lactic acid). It can be produced by either biotechnological fermentation or chemical synthesis, but the former route has received considerable interest recently, due to environmental concerns and the limited ...
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ژورنال
عنوان ژورنال: Microbial Cell
سال: 2021
ISSN: 2311-2638
DOI: 10.15698/mic2021.06.751